This Tumor Necrosis Factor Alpha ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat TNF-alpha. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat TNF-alpha and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat TNF-alpha, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat TNF-alpha. You can calculate the concentration of Rat TNF-alpha in the samples by comparing the OD of the samples to the standard curve.
Sample TypeSerum, plasma and other biological fluids
Incubation(s)3.5 hour(s)
Research AreasSignal Transduction, Metabolism, Cancer, Immunology, Microbiology, Cardiovascular
BackgroundThis ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat TNF-alpha. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat TNF-alpha and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat TNF-alpha, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat TNF-alpha. You can calculate the concentration of Rat TNF-alpha in the samples by comparing the OD of the samples to the standard curve.
Shipping ConditionShipped on cold gel packs.
Storage Condition and Shelf Life
Unopened kits should be stored at 2 to 8C until expiration. If opened, follow the instructions in the IFU. Generally speaking, if opened store the microplate, standard, detection antibody and HRP conjugate at -20C.
