• Multi-Species 5-HT (5-Hydroxytryptamine) ELISA Kit, Time 2.5 hours
1 1

5-HT (5-Hydroxytryptamine) ELISA Kit

5-HT (5-Hydroxytryptamine) ELISA Kit

This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with 5-HT protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to 5-HT. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of 5-HT in the samples is then determined by comparing the OD of the samples to the standard curve.

Catalog No: ELK7830
$376.00 USD
$376.00 USD
Sale Sold out
Packaging
2 weeks lead time
Download PDF

Product Details

Species Reactivity Multi-Species
Sensitivity 3.94 ng/mL
Detection Range 14.07-900 ng/mL
Sample Type serum, plasma, tissue homogenates
Incubation(s) 2.5h
Research Areas Signal Transduction, Infection immunity, Neuroscience, Gastroenterology
Shipping Condition Shipped on cold gel packs.
Storage Condition and Shelf Life If the kit is not opened, store the whole kit: 4°C(short time storage, valid for 6 months); -20°C(long-term storage, valid for 1 year). Avoid repeated freeze-thaw cycles If the kit is opened, Store the whole kit at 4°C. If the kit is not used up in 1 week. Store the Pre-Coated Microplate, Standard ,Biotinylated-Conjugate and Streptavidin-HRP at -20°C, the rest reagents at 4°C, please used up within 6 months.
Analyte Serotonin
Regulatory Status For Research Use Only
View full details

Inquire about this product