This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with 4-HNE protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to 4-HNE. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of 4-HNE in the samples is then determined by comparing the OD of the samples to the standard curve.
Sample Typeserum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Incubation(s)2.5h
Research AreasSignal Transduction, Metabolic pathway, Infection immunity, Hormone metabolism, Dermatology
Shipping ConditionShipped on cold gel packs.
Storage Condition and Shelf Life
If the kit is not opened, store the whole kit: 4°C(short time storage, valid for 6 months); -20°C(long-term storage, valid for 1 year). Avoid repeated freeze-thaw cycles
If the kit is opened, Store the whole kit at 4°C. If the kit is not used up in 1 week. Store the Pre-Coated Microplate, Standard ,Biotinylated-Conjugate and Streptavidin-HRP at -20°C, the rest reagents at 4°C, please used up within 6 months.
