Rat PINP (Procollagen I N-Terminal Propeptide) ELISA Kit
Rat PINP (Procollagen I N-Terminal Propeptide) ELISA Kit
With about 32 known citations, the Rat PINP (Procollagen I N-Terminal Propeptide) ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat Pâ… NP. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat Pâ… NP and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat Pâ… NP, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat Pâ… NP. You can calculate the concentration of Rat Pâ… NP in the samples by comparing the OD of the samples to the standard curve.
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