The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse IL-1Beta. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse IL-1Beta. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse IL-1Beta, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse IL-1Beta in the samples is then determined by comparing the OD of the samples to the standard curve.
Sample Typeserum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Incubation(s)3.5h
Research AreasCytokines, Tumor immunity, Infection immunity
Shipping ConditionShipped on cold gel packs.
Storage Condition and Shelf Life
If the kit is not opened, store the whole kit: 4C (short time storage, valid for 6 months); -20C (long-term storage, valid for 1 year). Avoid repeated freeze-thaw cycles
If the kit is opened, Store the whole kit at 4C . If the kit is not used up in 1 week. Store the Pre-Coated Microplate, Standard ,Biotinylated-Conjugate and Streptavidin-HRP at -20C , the rest reagents at 4C , please used up within 6 months.
