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Flag-Tag Mouse Monoclonal Antibody (2C5)

Flag-Tag Mouse Monoclonal Antibody (2C5)

The DYKDDDDK peptide (Flag-tag) is a small component of an epitope which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. It has been used extensively as a general epitope tag in expression vectors. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.A Flag-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a Flag-tag to this protein allows one to follow the protein with an antibody against the Flag sequence.

Catalog No: FY-IG0776
Regular price $239.00 USD
Regular price Sale price $239.00 USD
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Packaging
1.5 weeks
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Product Details

Application(s) WB, IF, IP
Species Reactivity N/A
Source Murine
Ab Type Monoclonal
Isotype IgG1
Immunogen Synthetic Peptide
Dilution WB 1:5,000 IHC 1:200 IF 1:2,000
Shipping Condition Shipped on cold gel packs.
Storage Condition and Shelf Life PBS, pH 7.4, containing 0.02% sodium azide and 50% glycerol. Store at -20°C. Avoid repeated freeze-thaw cycles. Stable for one year from the date of shipment.
Regulatory Status For Research Use Only
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